Chromatography of Commercial Analgesics - 878 Words

Chromatography of Commercial Analgesics Using the thin layer chromatography the major components of a commercial analgesic tablet will be indentified and purified. The active ingredients of the analgesic used were characterized by comparison with chromatograms of aspirin, acetaminophen, ibuprofen, mefanemic acid, and caffeine through the use of UV chromatography then the Rf value was calculated for each solvent. INTRODUCTION In this experiment, thin-layer chromatography (TLC) will be used to determine the composition of various analgesics. Chromatography is a technique used to separate and identify individual components in a mixture. Chromatographic techniques make use of the fact that components of a mixture tend to move at†¦show more content†¦This experiment aims to indentify and characterize the major components of commercial analgesic tablets which in this case the assigned tablet was Ibuprofen Alaxan. MATERIALS AND METHODOLOGY I. Preparing the TLC plates Cut a 5x10 TLC plastic sheet. six mark spots must be placed so that the solvents will be placed properly later on through the capillary, this marks must be 1cm apart and 1cm from the bottom edge of the TLC sheet. II. Preparing the Sample The sample analgesic was triturated with 2ml methanoltoluene it was then decanted and transferred into a vial. The standards that were used were already prepared, the aspirin, acetaminophen, ibuprofen, mefanemic acid, and caffeine. III. Sample Application Using a capillary tube, one end was dipped in the sample solution to allow the solution to enter by capillary action. On one end, using the index finger block the top-end of the capillary, lightly and then quickly spot it to the mark on the TLC plastic sheet. Using new capillary tubes, repeat the same process for the other spots for all. IV. Sample Developing The TLC plate was carefully placed inside a beaker containing ethylacetate-methan ol-aceticacid andShow MoreRelatedChromatography of Commercial Analgesics1861 Words   |  8 PagesChromatographic Analysis of a Commercial Analgesic’s Active Component I. Abstract This study on Chromatographic Analysis of a Commercial Analgesic’s Active Component seeks to separate, identify, quantify and analyze the active component found in Biogesic ®, which may be useful for public awareness despite its wide acceptance and availability in the market. Furthermore, this study applies thin layer chromatography (TLC) technique since it is the simplest, cheapest yet most effective approach in termsRead MoreParacetamol For Fight Fever And Pain927 Words   |  4 Pagescurrently due to its antipyretic and analgesic properties. The aim of the experiment is the production of paracetamol by formation of an amide bound between the amine group of p-aminophenol and the carbonyl group of acetic anhydride. Furthermore, drugs used in humans must pass Quality Control (QC) and Quality Assurance (QA) in order to ensure the purity of the substance. In the current experiment, methods such as melting point range analysis, Thin Layer Chromatography (TLC) and NMR spectrome try will beRead MoreEffects Of Anatomical Parts Of Ephedra Sinica1978 Words   |  8 Pages(Mahuanggen) of different plant samples. It was concluded that the pharmacologically important ephedrine alkaloids were present in higher quantities in the stems. This is why Ephedra herb, sold as dry stems of Ephedra sinica, has been used as a commercial source of ephedrine alkaloids worldwide, in particular Europe and America (Lee 2011). Ephedrine is a ÃŽ ²-phenylethylamine widely used as a sympathomimetic in herbal preparations. The structure of ephedrine possesses two chiral carbonsRead MorePansit Pansitan Research5452 Words   |  22 Pagesan active ingredient, will be more efficient and less expensive than the commercial hand soaps. Statement of the Problem 1. Can Pansit- pansitan liquid hand soap be as good as the commercial liquid hand soap in terms of: 2.1 total mean zone of inhibition? 2.2 reactivity rating? 2.3 inhibitory activity rating? 2. Is there a significant difference between Pansit-pansitan liquid hand soap and commercial liquid hand soap in terms of total mean zone of inhibition? 3. Is thereRead MoreHemp Cultivation in China42289 Words   |  170 Pagesthat, due its extremely low THC content, the THC content of the oil sent by the firm can only be determined by thin-layer chromatography or gas chromatography after special preparation. Since hemp seed oil and oils prepared from seeds not cleaned of leaf debris and tunics were found to have a cannabinoid content demonstrable by thin layer chromatography and gas chromatography (CBD was also identified), while oil prepared from seeds purified from the tepal (tunic) had none, it can be concluded thatRead MorePharmaeutical Industry Training Report with Deep Description of Different Area in Ibn Sina Pharmaceutical Industry Ltd.14426 Words   |  58 Pages | Introduction In Plant training is an opportunity to experience the industrial environment. This training is the bridge between the theoretical and practical in other words between institution and commercial organization. Industrial training in a pharmaceutical company completes the theoretical knowledge that we have studied for last four years. It is our pleasure to take the training in a well reputed company The IBN Sina Pharmaceutical